Fig. 3.

PDZ and LIM domain protein 5 (PDLIM5) is required for miR-17/20a-mediated suppression of PAI-1. A and B: human PASMCs were transfected with NC or miR-17/20a mimics (A) or inhibitors (B); 48 h later, cells were collected for measuring the expression of PAI-1. C and D: human PASMCs were transfected with NC or PDLIM5 siRNA (C) or PAI-1 siRNA (D); 48 h later, cells were collected for measuring PAI-1 and PDLIM5 expression levels. Representative blots and quantification are presented. E: human PASMCs were transfected with a control plasmid (pcDNA3) or a plasmid encoding PDLIM5-V5-His; 48 h later, cells were collected for measuring the expression of PAI-1. Data are presented as means ± SE. *P ≤ 0.05, **P ≤ 0.01; n = 5.