Fig. 8. Anterior-posterior axis is established before the left-right axis in the development of the brain.

(A) Dorsal view of the neural tube and superior view of the adult brain in mouse. The primary brain vesicles in the neural tube and their corresponding structures in adult brain are shown. (B,C) Calculated trees based on hgRNA barcodes in two adult mice. See fig. S12 for the full barcodes. (D) Distribution of tree calculation outcomes for mouse 2 when only including $m$ of the n hgRNAs in each mouse (${\displaystyle }{\text{⬚}}^n{C}_m$ combinations). Only hgRNAs with at least 7% mutation rate in one of the samples were considered. (E) Impact Score of each hgRNA in the early lineage tree of mouse 2.