OST1 Mediates BR-induced Stomatal Closure.
(A) Stomatal aperture in the ost1 mutant in the ABA-induced stomatal closure assay. Result is representative of two independent experiments (n ≥ 30 stomata per genotype).
(B) Stomatal apertures in the ost1 mutant in the BR-induced stomatal closure assay. Result is representative of two independent experiments (n ≥ 30 stomata per genotype).
(C) and (D) Stomatal apertures of OST1-overexpressing plant in the ABA- or BR-induced stomatal closure assay. The ABA (C) and BR (D) responsiveness of wild-type Col-0, cdl1, 35S-OST1-myc, and 35S-OST1-myc cdl1 plants was compared. Result is representative of two independent experiments (n ≥ 30 stomata per genotype).
Detached rosette leaves were incubated in stomatal closure solution containing mock, ABA, or BL as indicated for 2 h. Error bars indicate se. Statistical significant differences are indicated by different lowercase letters (two-way ANOVA, P < 0.005).