Figure 13.

Determination of H₂O₂ Level during Photoautotrophic N Deprivation.

(A) Extracellular H₂O₂ level determined by Amplex Red.

(B) Relative DCF fluorescence level.

(C) Intracellular ROS level determined using H2DCFDA staining.

Cells were cultivated under constant light in photoautotrophic conditions with additional supply of 2% CO₂ in the air. Values are the mean of biological replicates (i.e., independent shaking flask cultures; n = 4, sd). Asterisks indicate significant difference compared with control strains (dw15 and C1) by paired-sample Student’s t test (*P < 0.05). C1, the complemented line.