Figure 5.

mdh2-2 Overaccumulates TAG and Starch, and Possesses Higher Photosynthetic Activity during Photoautotrophic N Deprivation.

(A) TAG content.

(B) Starch content.

(C) PSII operating efficiency.

(D) LD imaging after cells being stained by BODIPY.

Cells were cultivated under constant light in photoautotrophic conditions with additional supply of CO₂ at 2% in the air. Values are the mean of biological replicates (i.e., independent shaking flask cultures; n = 6, sd). Actinic light (200 µmol m⁻² s⁻¹) supplied by a red LED source was used for PSII yield measurement. Asterisks indicate statistically significant changes compared with the parental strain (CC4533) by paired-sample Student’s t test (**P ≤ 0.01). Cells were stained with BODIPY, and pseudocolors were used: lipid droplet in green and chlorophyll in red. mag., magnification.