Figure 2.

HIL1 Has Relatively Unique Characteristics among the Lipase Genes.

(A) Schematic representation of HIL1 structure. Regions of the putative cTP sequence, C2 domain, and lipase-like domain are shown in green, blue, and brown boxes, respectively.

(B) Sequence similarity between HIL1 and DAD1-like proteins. Arabidopsis genes that exhibit homology to the lipase-like domain of HIL1 were selected. Only the lipase-like domain sequences were used to generate the amino acid sequence alignment (Supplemental Data Set 1). Twelve DAD1-like proteins formed a group (blue). HIL1 is separated from this group (purple).

(C) Phylogenetic relationships among HIL1 and homologs in terrestrial plants. The phylogenetic tree was constructed from an alignment of deduced full-length amino acid sequences (Supplemental Data Set 1). The bar indicates amino acid substitutions per site. Bootstrap values (2000 replicates) > 70% are shown next to the branches.