Figure 5.

Profiles of Characteristic Glycerolipid Species in Leaves of the Wild-Type, T-DNA Insertion Mutant, and Complemented Plants under Heat Stress.

Each data point of 34:6-MGDG (A), 34:6-DGDG (B), 36:6-PC (C), 36:6-PE (D), and 54:9-TAG (E) is represented by a box and whisker plot. Seventeen-day-old wild-type (WT), hil1 mutant, and hil1/HIL1-complemented plants were treated with 38°C for 1 d (1d38C WT, 1d38C hil1, and 1d38C hil1/HIL1). Crude lipid extracts from leaves were analyzed by LC-MS in the positive ion mode. Plants grown continuously at 22°C were examined as the normal growth condition (1d22C WT, 1d22C hil1, and 1d22C hil1/HIL1). Each data point expresses biological replicates of n ≥ 20. The #3, #6 and #9 hil1/HIL1-complemented plants were examined (Supplemental Data Set 2). The whole rosettes harvested from two to three different plants were pooled as one biological repeat. P values of Welch’s t test with < 0.001 (asterisk) are shown on each genetic variable under heat stress. The profiles of the other lipid species are shown in Supplemental Figure 3. Statistical results are in Supplemental Data Set 3.