Table 2. Determination of Lipase Substrate of the HIL1 Recombinant Protein.
| Substrate | MBP-mHIL1 | mHIL1 Removed MBP-Tag |
|---|---|---|
| MGDG | 43.1 ± 1.7 | 29.1 ± 0.8 |
| Hydrogenated MGDG | N.D. | N.D. |
| DGDG | N.D. | N.D. |
| SQDG | 0.1 ± 0.0 | 0.3 ± 0.1 |
| 36:6-PG (18:3/18:3) | 1.8 ± 0.6 | 2.3 ± 0.6 |
| 36:0-PG (18:0/18:0) | N.D. | N.D. |
| 36:6-PC (18:3/18:3) | N.D. | N.D. |
| 34:2-PC (16:0/18:2) | N.D. | N.D. |
| 16:0-lyso-PC (16:0/OH) | N.D. | N.D. |
| 36:6-PE (18:3/18:3) | 26.4 ± 0.4 | 23.3 ± 0.6 |
| 34:1-PE (16:0/18:1) | N.D. | N.D. |
| 36:6-DAG (18:3/18:3) | N.D. | N.D. |
| 54:9-TAG (18:3/18:3/18:3) | N.D. | N.D. |
Data are presented as μmol min−1 mg−1 protein. Each substrate was incubated with the purified MBP-mHIL1 recombinant protein (0.2 μg) or mHIL1 protein removed MBP-tag (0.2 μg). Lipids were analyzed by LC-MS. The decreases in substrate were quantified in the positive ion mode. Each data point represents the mean value ± sd (n = 3). N.D., not detected.