Figure 1.

Embryo-Specific Expression of pdNAD-MDH to Rescue the Embryo Lethality of pdnad-mdh Plants.

(A) Schematic diagram of the P_ABI3:pdNAD-MDH-YFP and P_pdNAD-MDH:pdNAD-MDH-YFP constructs. For the former construct, the ABI3 promoter (P_ABI3) was placed upstream of the pdNAD-MDH coding sequence fused at its C terminus to YFP. The latter construct was described by Beeler et al. (2014).

(B) Opened siliques of pdNAD-MDH^+/− plants transformed with the P_ABI3:pdNAD-MDH-YFP construct. Siliques from the wild type (Ler), untransformed (UT) pdNAD-MDH^+/− plants, and pdNAD-MDH^+/− transformed with the P_pdNAD-MDH:pdNAD-MDH-YFP construct are shown for comparison. White seeds are indicated with an arrow. Bar = 1 mm.

(C) Expression of the P_ABI3:pdNAD-MDH-YFP construct in Arabidopsis embryos after transformation of pdNAD-MDH^+/− plants. Expression was detected by fluorescence microscopy on embryos at three different developmental stages (globular, heart, and torpedo). Bar = 50 µm.