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. 2018 Jun 22;30(8):1745–1769. doi: 10.1105/tpc.18.00121

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Figure 10. — ITC Thermograms of NADH Titrated into the pdNAD-MDH Wild-Type and Inactive Proteins.

(A) ITC profile of NADH injected into a solution of recombinant His-pdNAD-MDH wild-type protein at 25°C. The upper panel shows the raw calorimetric data. The plot below shows the integrated enthalpy as a function of the NADH/pdNAD-MDH molar ratio. Reactions are exothermic.

(B) to (D) Experiments were conducted as described for (A) with the His-pdNAD-MDH R162Q protein (B), the His-pdNAD-MDH R234Q protein (C), and the His-pdNAD-MDH R162Q R234Q protein (D).