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Immunoblot and Native-PAGE Detection of pdNAD-MDH in Ler, miR-mdh-1, and pdnad-mdh−/− Seedlings Rescued with the PABI3:pdNAD-MDH-YFP Construct.
(A) Immunoblots were conducted with the pdNAD-MDH antibody. Equal amounts of soluble protein (5 µg) were loaded. The migration of molecular weight markers is indicated (left).
(B) In-gel activity assay of the NAD-MDH. The two bands corresponding to pdNAD-MDH activity are indicated (arrows, right). Equal amounts of protein (15 µg) were loaded.
