Figure 5.

Etiolated Growth of miR-mdh-1 and Etioplast Structure.

(A) Wild-type (Col) and miR-mdh-1 seedlings were grown on 0.5× strength MS agar plates in different diel light conditions (24 h dark, 12 h light/12 h dark, 24 h light). Bar = 1 cm.

(B) Quantification of the hypocotyl length of etiolated wild-type and miR-mdh-1 seedlings. Values represent the mean ± se of measurements conducted on n = 89 and n = 86 seedlings for the wild type and miR-mdh-1, respectively.

(C) Etioplast ultrastructure in cotyledons of etiolated wild-type and miR-mdh-1 seedlings observed by transmission electron microscopy. Bars = 500 nm

(D) Proportion of etioplasts showing normal, compromised, or absent prolamellar body structures in cotyledons of the wild type and miR-mdh-1. The first 139 and 256 etioplasts, observed in wild-type and miR-mdh-1 cotyledons, respectively, were categorized based on their prolamellar body structure.