Figure 7.

Complementation Test with Various NAD-MDH Isoforms Expressed in pdnad-mdh.

(A) Constructs encoding NAD-MDH isoforms under the control of the pdNAD-MDH promoter. The plastidial transit peptide from Rubisco small subunit was fused to the N terminus of each isoform. The constructs shown are tagged at the C terminus with YFP. Similar constructs were cloned with the Flag-HA tag in place of YFP.

(B) Genotyping results of the T2 progeny from T1 plants heterozygous for the pdnad-mdh mutation and expressing the different NAD-MDH isoforms. Numbers above the bars indicate the number of BASTA-resistant T2 plants that were genotyped.

(C) Chloroplast localization of the NAD-MDH isoforms. Similar constructs to those shown in (A), except with the 35S promoter in place of the native promoter, were transiently expressed in Nicotiana benthamiana leaves and imaged using confocal microscopy. Bar = 5 µm.