Table 2. Proteins Identified in Anti-YFP Immunoprecipitates from Two Independent Lines Overexpressing FtsH12-YFP.
Accession No. | Identified Proteins (236/247) | PUBQ10:FtsH12-YFP | |
---|---|---|---|
1 | 2 | ||
AT1G79560 | FtsH protease 12, Embryo defective 1047, Embryo defective 156, Embryo defective 36 | 386 | 371 |
ATCG00860 | Chloroplast Ycf2 | 181 | 237 |
AT3G04340 | FtsH extracellular protease family, FtsHi 5, Embryo defective2458 | 247 | 179 |
ATCG01130 | Chloroplast Ycf1, TIC214 | 70 | 111 |
AT3G16290 | FtsH extracellular protease family, FtsHi 2, Embryo defective 2083 | 46 | 86 |
AT5G64580 | FtsH extracellular protease family, FtsHi 4, Embryo defective 3144 | 66 | 83 |
AT4G23940 | FtsH extracellular protease family, ARC1, FtsHi1 | 69 | 52 |
AT1G01320 | Tetratricopeptide repeat-like superfamily protein | 6 | 32 |
AT4G02510 | Translocon at the outer envelope membrane of chloroplasts 159 | 37 | 31 |
AT5G53860 | Embryo defective 2737 | 13 | 26 |
ATCG00480 | ATP synthase subunit beta | 8 | 23 |
AT3G47520 | pdNAD-MDH | 5 | 22 |
AT1G07920 | GTP binding Elongation factor Tu family protein | 15 | 20 |
AT1G43170 | Ribosomal protein 1 | 5 | 20 |
ATCG00120 | ATP synthase subunit alpha | 42 | 19 |
ATCG00490 | Ribulose-bisphosphate carboxylase | 48 | 16 |
AT5G01590 | Histone-lysine N-methyltransferase ATXR3-like protein | 14 | 16 |
AT2G41840 | Ribosomal protein S5 family protein | 6 | 16 |
The IPs were conducted with anti-YFP beads on extracts of material harvested from rosette leaves, and the coeluting proteins were identified using LC-MS/MS. Values represent the total spectrum count of peptides matching each protein. Proteins found in the control sample (IPs from wild-type plants) were assumed to be contaminants and excluded. The full data set can be found in Supplemental Data Set 1.