Table 3. Proteins Identified in IPs of YFP-Tagged NAD-MDH Proteins (AtpdNAD-MDH, AtcyMDH1, ScmMDH1, AtmMDH1, and AtpMDH) Expressed in Heterozygous pdnad-mdh Plants.
| Accession No. | Identified Proteins (102/115) | pdnad-mdh+/− | ||||
|---|---|---|---|---|---|---|
| At pdNAD-MDH | At cyMDH1 | Sc mMDH1 | At mMDH1 | At pMDH1 | ||
| GFP_AEQVI | YFP-tag | 108 | 7 | 3 | 66 | 162 |
| AT3G47520 | pdNAD-MDH | 142 | 3 | 0 | 18 | 6 |
| AT1G04410 | cyMDH1 | 0 | 8 | 5 | 0 | 0 |
| AT1G53240 | mMDH1 | 4 | 0 | 0 | 96 | 0 |
| AT2G22780 | pMDH1 | 0 | 2 | 2 | 0 | 312 |
| ATCG00860 | Chloroplast Ycf2 | 193 | 0 | 0 | 4 | 0 |
| AT3G04340 | FtsH extracellular protease family, FtsHi 5, Embryo defective2458 | 192 | 0 | 0 | 0 | 0 |
| AT1G79560 | FtsH protease 12, Embryo defective 1047, Embryo defective 156, Embryo defective 36 | 155 | 0 | 0 | 4 | 0 |
| AT5G64580 | FtsH extracellular protease family, FtsHi 4, Embryo defective 3144 | 102 | 0 | 0 | 0 | 0 |
| AT3G01510 | Like SEX4 1 | 82 | 0 | 0 | 0 | 0 |
| AT4G23940 | FtsH extracellular protease family, ARC1, FtsHi1 | 69 | 0 | 0 | 0 | 0 |
| ATCG01130 | Chloroplast Ycf1 protein | 59 | 0 | 0 | 0 | 0 |
| AT3G16290 | FtsH extracellular protease family, FtsHi 2, Embryo defective 2083 | 54 | 0 | 0 | 0 | 0 |
| AT5G53860 | Embryo defective 2737 | 29 | 0 | 0 | 0 | 0 |
| AT4G02510 | Translocon at the outer envelope membrane of chloroplasts 159 | 29 | 0 | 0 | 0 | 0 |
IPs were conducted with anti-YFP beads on extracts of rosette leaves, and the coeluting proteins were identified using LC-MS/MS. Values represent the total spectrum count of peptides matching each protein. Proteins also identified in the control sample (Ler) were assumed to be contaminants and excluded. The full data set can be found in Supplemental Data Set 1.