Table 4. Proteins Identified in Anti-HA Immunoprecipitates from Homozygous pdnad-mdh Plants Expressing Noncatalytic Versions of pdNAD-MDH.
Accession No. | Identified Proteins (917/929) | pdnad-mdh−/− | |||
---|---|---|---|---|---|
pdNAD-MDH WT | pdNAD-MDH R162Q | pdNAD-MDH R234Q | pdNAD-MDH R163Q R234Q | ||
AT3G47520 | pdNAD-MDH | 96 | 49 | 31 | 22 |
ATCG00860 | Chloroplast Ycf2 | 283 | 457 | 332 | 405 |
AT3G04340 | FtsH extracellular protease family, FtsHi 5, Embryo defective2458 | 408 | 330 | 210 | 362 |
AT1G79560 | FtsH protease 12, Embryo defective 1047, Embryo defective 156, Embryo defective 36 | 373 | 309 | 212 | 317 |
AT5G64580 | FtsH extracellular protease family, FtsHi 4, Embryo defective 3144 | 221 | 207 | 164 | 159 |
AT3G16290 | FtsH extracellular protease family, FtsHi 2, Embryo defective 2083 | 125 | 228 | 158 | 179 |
ATCG01130 | Chloroplast Ycf1 | 70 | 166 | 174 | 223 |
AT4G23940 | FtsH extracellular protease family, ARC1, FtsHi1 | 153 | 141 | 153 | 109 |
AT4G02510 | Translocon at the outer envelope membrane of chloroplasts 159 | 25 | 95 | 113 | 133 |
AT3G01510 | Like SEX4 1 | 159 | 58 | 105 | 23 |
AT5G53860 | Embryo defective 2737 | 30 | 54 | 55 | 54 |
The IPs were conducted with anti-HA beads on extracts of rosette leaves, and the coeluting proteins were identified using LC-MS/MS. Values represent the total spectrum count of peptides matching each protein. Proteins found in the control sample (IPs from wild-type [Ler] plant extracts) were assumed to be contaminants and excluded. The full data set can be found in Supplemental Data Set 1.