Figure 3.

Analysis of Polysome Association of the Plastid Transcripts Encoding ATP Synthase Subunits.

Total leaf extracts from wild-type, bfa1-1, and cgl160 plants were fractionated by sucrose density gradient centrifugation (15–55%) under native conditions. After centrifugation, the sucrose gradients were divided into 10 fractions of equal volume for RNA isolation and analysis, as noted above the gels. The RNAs were blotted and probed with DIG-labeled DNA probes corresponding to the plastid atpA, atpF, atpH, and atpI (A) as well as atpE (B) transcripts. rRNAs detected by ethidium bromide staining were used as fractionation and loading controls (C). 23S*, two breakdown products of the chloroplast 23S rRNA. Numbers to the right of the gel indicate sedimentation coefficients of the major RNAs.