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. 2018 Jul 31;30(8):1924–1942. doi: 10.1105/tpc.18.00042

Figure 7.

Figure 7.

pMdhpRNA277-GD Is Induced by ALT1 Infection in Transgenic Apple, but pMdhpRNA277-HF Is Not.

(A) Schematic of the T1 construct used to stably overexpress MdhpRNA277 under the control of the pMdhpRNA277-GD promoter in GL-3 lines.

(B) RNA gel blot analysis of mdm-siR277-1/2 content at 48 HPI in GL-3 transgenic lines GL1-1(-8) compared with CK.

(C) Disease symptoms in 4-week-old GL1-1(-8) lines at 48 HPI.

(D) Disease rates in 4-week-old GL1-1(-8) lines at 48 HPI.

(E) Schematic of construct used to stably overexpress MdhpRNA277 under the control of the pMdhpRNA277-HF promoter in the GL-3 lines.

(F) RNA gel blot analysis of mdm-siR277-1/2 content at 48 HPI in GL-3 transgenic lines GL2-1(-8) compared with CK.

(G) Disease symptoms in 4-week-old GL2-1(-8) lines at 48 HPI.

(H) Disease rates in 4-week-old GL2-1(-8) lines at 48 HPI. Spore inoculum concentration: 2 × 105 cfu/mL. Spore growth was measured at 6 DPI. Error bars represent standard deviations calculated from three biological replicates (separate biological material) were performed for each treatment. Student’s t test: **P < 0.01.