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. 2018 Sep 10;30(8):1943–1945. doi: 10.1105/tpc.18.00475

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Supplemental Figure 6. — Corrected: Overexpression of DN-RabA2A Induces Aggregates of PIN2 but Not PIN1.

(A) and (C) The 20 µM DEX induction does not change the PIN1 protein localization.

(B) and (D) The 20 µM DEX-induced overexpression of DN-RabA2A does not change the PIN1 protein localization.

(E) and (G) The 20 µM DEX induction does not change the PIN2 protein localization.

(F) and (H) The 20 µM DEX-induced overexpression of DN-RabA2A significantly increases PIN2 internalization as visualized by intracellular vesicles.

(I) to (K) Stable overexpression of DN-RabA2A does not change PIN1 polarity.

(L) to (N) Stable overexpression of DN-RabA2A results in enhanced PIN2 agglomerations, which are colocalized with the DN-RabA2A vesicles (white arrowheads). Images are representative of three repeats. Bars = 10 µm. Note: Original panels (E) to (J) are relabeled (I) to (N).