Figure 6.
CD28 influences CD3ζ phosphorylation (Phos) kinetics. (A) A schematic of the His-tagged CD28-CD3ζ recombinant protein is shown. (B) Experimental data (circles) and model fit (lines) for CD3z ITAM phosphorylation on wild-type CD28-CD3ζ, CD28-Y206F-CD3ζ, and CD28-Y209F-CD3ζ are shown. Error bars represent the SD of two technical replicates normalized by site-specific standard curves. (C) Estimated parameter sets are shown. Solid bars show the mean and SD of the 50 best fitted parameter sets. Shaded bars show the value of parameters that were held constant during fitting. For all model fits, the value of XI was held constant at 0.115, based on the best fitted value from the CD3ζ-only model fits. To see this figure in color, go online.