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. 2018 Sep 17;13:152. doi: 10.1186/s13023-018-0886-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Neuronal cell differentiation from Tay-Sachs iPSCs. a Immunofluorescence staining of TSD and WT neurons. MAP2, β-III-tubulin and Neurofilament-L are neuronal cell protein markers while Nestin is a neural stem cell marker, serving as the negative control. b Nile red staining on TSD and WT neurons. c LysoTracker red staining on TSD and WT neurons. No significant increase of Nile red staining or LysoTracker red staining was observed in TSD neurons compared to the WT control cells. Data are displayed as mean ± SD. * p < 0.05, compared to the WT control. IxA, integrated cell intensity