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Primary and secondary antibodies used and their dilutions.
| Antigen | Species | Dilution | Provider |
|---|---|---|---|
| Oestrogen receptor (ER) alpha | Rabbit | 1 : 100 | RM-9101 (Neomarkers, Fremont, CA, USA) |
| Progesterone receptor (PR) | Rabbit | 1 : 100 | RM-9102 (Neomarkers, Fremont, CA, USA) |
| Androgen receptor (AR) | Mouse | 1 : 25 | Ab9474 (Abcam, Cambridge, UK) |
| Anti-rabbit IgG | Mouse | 1 : 1000 | RG-96 (Sigma-Aldrich, St. Louis, MO, USA) |
| Anti-mouse IgG | Goat | 1 : 300 | Polyclonal (Sigma-Aldrich, St. Louis, Missouri, USA) |
