Figure 1.

Kinase activity of CaMKII is basically the same between the hippocampus and amygdala in the wild-type mouse and in the kinase-dead CaMKIIα (K42R)-KI mouse. Left, Total and Ca²⁺/calmodulin-independent autonomous activity of CaMKII in homogenates from the hippocampus and amygdala of wild-type (WT) mice. These values reflect the summation of the activity of CaMKIIα and CaMKIIβ, the two major CaMKII isoforms in the brain. Right, Kinase activity of CaMKII in homogenates from the hippocampus and amygdala of kinase-dead homozygous CaMKIIα (K42R)-KI mice. These values reflect the activity of intact CaMKIIβ. Note that figures in percentages above the columns represent relative activity against the value of corresponding control wild-type activity. The total activity was measured in the presence of Ca²⁺/calmodulin. The Ca²⁺/calmodulin-independent autonomous activity was measured in the absence of Ca²⁺/calmodulin. No significant difference was observed in the activity between the hippocampus and amygdala in both genotypes (see text in detail). Open columns, total activity; filled columns, Ca²⁺/calmodulin-independent autonomous activity. Error bars indicate SEM. Hippocampus, n = 6; amygdala, n = 6 for each genotype.