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. 2018 Sep 13;5(4):ENEURO.0270-18.2018. doi: 10.1523/ENEURO.0270-18.2018

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Copyright © 2018 Cissé et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 2. — Fluorescent images of ACh recorded and Nb-labeled cells. Neurons which were recorded and labeled with Nb (in red) were identified as ACh neurons by immunofluorescent staining for VAChT or ChAT (in blue). A, Located in the LDT of a TG-A mouse (ChAT8), an Nb-labeled cell, which was VAChT+, appeared to express ChR2-EYFP (evident as YFP over the plasma membrane). B, Located in the LDT of a TG-UA mouse (Chronic (C)ChAT8), an Nb-labeled cell, which was ChAT+, appeared to express ChR2-EYFP (revealed by immunohistochemical staining for GFP over the plasma membrane). C, Located in the PPT of a TG-UA mouse (CChAT13), an Nb-labeled cell, which was ChAT+, also appeared to express ChR2-EYFP (revealed by immunohistochemical staining for GFP over the plasma membrane). D, Located in the LDT of a WT-UA mouse (CWT8), an Nb-labeled cell, which was ChAT+ was also confirmed to be immunonegative for GABA.