L-selectin is dynamically regulated after SCI. A, Flow cytometry gating of leukocytes and leukocyte subsets in the peripheral blood at 24 and 72 h post-SCI. B, Representative flow cytometry histograms for CD62L staining of myeloid cells in the peripheral blood from uninjured mice (gray line) and injured mice at 24 h (red line) and 72 h (blue line) post-SCI. Representative isotype staining is shown in solid black. C, L-selectin (CD62L) levels on myeloid lineage cells in the peripheral blood from uninjured WT mice, and injured WT mice at 24 and 72 h post-SCI. N = 4 for UN, N = 8 for 24 h, and N = 6 for 72 h. One-way ANOVA with Tukey’s post hoc test (p = 0.004, <0.0001, 0.046, and 0.0001 and F(2,15) = 7.97, 35.8, 3.80, and 17.7, respectively). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.