Figure 5.

Leukocyte accumulation is transiently reduced in KO mice compared to WT mice after SCI. A, B, Flow cytometry gating of leukocytes and leukocyte subsets in the injured spinal cord at A, 24 h and B, 72 h post-SCI. C, Accumulation of myeloid cells (CD11b⁺) and neutrophils (Ly6C^low/Ly6G⁺), as determined by flow cytometry, was reduced in L-selectin KO compared to WT mice at 24 h post-SCI (*p = 0.028 and 0.038, respectively). There were no differences in infiltrating inflammatory (Ly6C^hi/Ly6G^-, p = 0.10) or non-classical monocyte subsets (Ly6C^low/Ly6G^-, p = 0.72) between WT and KO mice. N = 7 for WT and N = 8 for KO. Mann–Whitney test. D, There was no difference in the accumulation of any leukocyte subtype at 72 h post-SCI. N = 6/group. Unpaired two-tailed Student’s t tests. p = 0.82, 0.81, 0.81, and 0.45 and t₍₁₀₎ = 0.23, 0.25, 0.24, and 0.79, respectively. E, There were no differences in microglia (CD11b⁺/CD45^low/Ly6G^-) between WT and KO mice at 24 or 72 h post-SCI (p = 0.10 and 0.38, respectively). F, Prevalence of myeloid lineage subsets in the peripheral blood was similar between WT and KO mice at 24 and 72 h post-SCI. N = 7–8/genotype. Unpaired two-tailed Student’s t tests. No differences were detected (p = 0.68, 0.25, 0.80, and 0.70 and t₍₁₃₎ = 0.42, 1.19, 0.25, and 0.40, respectively).