Figure 1.

Expression of hM3Dq in the LH. A, Schematic of the AAV used. B, AAV-injected Orexin-tTA mice exhibited widespread neuronal expression of hM3Dq-mCherry despite the presence of DOX in the chow. C, Despite the absence of tTA, Gad2-IRES-Cre;R26R-EYFP mice injected with AAV-TetO-hM3Dq-mCherry exhibited a pattern of hM3Dq-mCherry expression that was similar to DOX-fed Orexin-tTA mice. Scale bar in B, C = 250 µm. Numbers in the upper left in C denote approximate AP distance from bregma (mm). D, Photomicrographs showing GAD2-EYFP⁺ neurons lateral to the fornix (Fx, left), hM3Dq-mCherry transfected neurons (center) and double-labeled neurons in yellow after merging both images (right). Scale bar = 100 µm. M and L denote medial and lateral. E, Photomicrographs showing ADA⁺ neurons in TMN (left), hM3Dq-mCherry transfected neurons (center), and merged image (right) showing the absence of double-labeled neurons. Scale bar = 50 µm.