Figure 4.

Hcrt signaling is not required for LH activation-induced arousal. Upper panels show hourly percentage of time spent in W (A), NREM (B), and REM sleep (C) and lower panels (A’–C’) show corresponding mean hourly bout duration for each state after injection of either VEH or ALM during the inactive phase at ZT4 followed by either SAL or CNO at ZT5. Arrows at the bottom left of the abscissa in A’–C’ indicate the times of dosing. Symbols above each time point denote significant hourly differences between the indicated treatments according to the legends in panels A, A’ when a significant treatment × time interaction was found (p < 0.001, n = 10, two-way repeated measures ANOVA, Bonferroni post hoc t test).