Figure 7.

c-FOS activity in the SuM does not increase after CNO treatment. A, c-FOS expression in the mammillary region after treatment with SAL followed by 90 min of SD. B, c-FOS expression in the SuM after CNO treatment showing similar c-FOS expression than SAL treatment. C, mCherry immunohistochemistry for the region depicted in B showing hM3Dq expression only in the lateral regions and almost no labeling in the medial aspect of SuM. Black dotted lines indicate the approximate region used for counting c-FOS in the SuM. LM, lateral mammillary nucleus; MM, medial mammillary nucleus. Scale bar = 250 μm.