Fig. 6.

TbArl3A and TbArl3C are critically involved in flagellum biogenesis. (A–C) Inducible expression of the GTP-locked mutant forms of TbArl3A (Q70L) and TbArl3C (Q77L) both severely inhibited flagellum biogenesis in PCF cells. TbArl3B (Q71L), however, has no detectable effects. Anti-PFR1 and L3B2 antibody were used to mark the flagella and FAZ, respectively. Scale bar: 10 μm. (D–F) Growth curves of cells in the absence (Tet−) or presence (Tet+) of tetracycline for inducible expression of TbArl3 mutants. Growth curves shown are representative of two independent experiments. (G) Immunoblots confirm the expression of all three mutant TbArl13 homologs.