Fig. 4.

Hsp70 inhibition induces autophagy but not apoptosis in RMS13-R cells. (A) RMS13-R and RMS13 cells were treated with 7.5 µM MAL3-101 for the indicated times and lysates were prepared and immunoblotted for LC3B, a marker for autophagic flux, and for indicators of apoptosis (PARP, cleaved caspase-8, cleaved caspase-3 and CHOP). (B) The corresponding fold increase of the indicated apoptotic and autophagy associated markers in RMS13-R (black) and RMS13 (white) cells relative to the DMSO control are plotted as the mean±s.e.m. (n=8 for LC3B, CHOP, and cleaved PARP; n=3 for cleaved caspase-3; n=2 for cleaved caspase-8). *P<0.05, **P<0.005, ***P<0.0001.