Fig. 3.

Loss of SCML2 leads to ectopic patches of DAPI-stained chromatin in round spermatids. (A-C) Immunostaining of testicular paraffin sections with the indicated antibodies. Images were acquired with a confocal microscope. Boxed regions are magnified in the right panels. CC, chromocenter; PMSC, postmeiotic sex chromatin. Arrows indicate ectopic patches in Scml2-KO RSs. Scale bars: 20 µm (left) and 5 µm (right). Three independent pairs of wild-type and Scml2-KO mice were examined and representative images are shown.