Fig. 5.

Loss of SCML2 leads to ectopic expression of somatic lamins in round spermatids. (A) Distribution of RNA-seq and ChIP-seq reads at the Lamin gene locus. Somatic lamins (LmnaA and LmnaC) were derepressed at the transcriptional level in round spermatids of Scml2-KO. A summary of A- and B-type lamins is shown on the right. (B) Western blotting with anti-Lamin antibodies using purified pachytene spermatocytes (PSs) and round spermatids (RSs) from wild-type and Scml2-KO mice. (C) Immunostaining of testicular paraffin sections with the indicated antibodies. Images were acquired with a confocal microscope. Boxed regions are magnified in the right panels. CC, chromocenter; PMSC, postmeiotic sex chromatin. Arrows indicate ectopic patches in the Scml2-KO RSs. Scale bars: 20 µm (left) and 5 µm (right). Three independent pairs of wild-type and Scml2-KO mice were examined and representative images are shown.