Fig. 6.

SCML2 is required for CENP-V localization on pericentromeric heterochromatin in round spermatids. (A) Immunostaining of CENP-V in slides that preserve the relative nuclear structure of spermatogenic cells. Pairs of round spermatids are shown. CC, chromocenter; PMSC, postmeiotic sex chromatin. Images were acquired with a wide-field microscope. Scale bars: 10 µm. Three independent pairs of wild-type and Scml2-KO mice were examined and representative images are shown. (B-D) Summary of action of SCML2 in spermatogenesis. (E) Schematic of round spermatid nuclei in wild-type and Scml2-KO mice.