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. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: Chem Soc Rev. 2018 Jul 30;47(15):5646–5683. doi: 10.1039/c7cs00263g

Fig. 23.

Fig. 23

a) Schematic illustration of the heterogeneous immunoassay in alginate microparticles.240 Reprinted with permission from ref. 240. Copyright 2014, The Royal Society of Chemistry. b) Analytical procedure for single-cell forensic STR typing, including encapsulation of single cells and DNA isolation, STR target amplification and STR products analysis.243 Reprinted with permission from ref. 243. Copyright 2014, American Chemical Society. c) Platform for DNA barcoding thousands of Cells.244 Cells are encapsulated into droplets with lysis buffer, reverse-transcription mix, and hydrogel microspheres carrying barcoded primers. After encapsulation, primers are released. cDNA in each droplet is tagged with a barcode during reverse transcription. Droplets are then broken and material from all cells is linearly amplified before sequencing. Reprinted with permission from ref. 244. Copyright 2015, Elsevier.