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. 2018 Oct;24(10):1403–1417. doi: 10.1261/rna.065482.117

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© 2018 Sarin et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — PGC and C18 capillaries enable the analysis of ribonucleosides in nLC ESI-MS/MS. (A) Representative total ion chromatograms (TIC) of 100 ng of the LSM or CSM (C18 only) analyzed on nanoflow capillary columns packed with PGC-A (left panel), PGC-B (middle panel), or C18 (right panel) material. (B) Extracted ion chromatograms (XIC) for adenosine (A), cytidine (C), guanosine (G), uridine (U), pseudouridine (Ψ), inosine (I), N⁴-acetylcytidine (ac⁴C), 1-methyladenosine (m¹A), 2′-O-methyladenosine (Am), and N⁶-methyladenosine (m⁶A; CSM only). (C) Example MS1 spectra recorded at the retention times corresponding to the nucleosides analyzed in B. (NL) Normalized target level.