FIGURE 4.

Comprehensive analysis of highly complex analytes is achieved using PGC-B. (A) Representative TIC chromatograms of 31 ribonucleoside standards (100 ng of CSM, Supplemental Table 1; left panels) and 250 ng of an enzymatic digest of bulk tRNA isolated from Saccharomyces cerevisiae strain BY4741 (right panels) analyzed on a nano PGC-B capillary column. (B) Overlay of the XICs for all CSM ribonucleosides detected in the respective samples. (C) XICs of selected nucleosides present in the samples. Note the capability of the PGC material to separate, with the exception of methylated guanosine, all positional isomers. (NL) Normalized target level.