Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct;24(10):1305–1313. doi: 10.1261/rna.067033.118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Wang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 2. — Q modification and tRNA fragments in vivo by RNA-seq. (A) Comparison of the tRNA isoacceptor family abundance of 0Q and 100Q HEK293T cells by DM-tRNA-seq. Arrows indicate the four Q-modified cognate tRNAs. The total number of assigned tRNA reads is set to 1 in each sample, and the fraction of each tRNA isoacceptor is shown. (B) Heatmap of tRNA fragment abundance at the isoacceptor family level. The amount of all tRNA fragment products of all sizes in each sample are added together and set as 1; shown are the fractions of individual tRNA isoacceptors. Arrows indicate the four Q-modified cognate tRNAs. The scale bar shows the fraction tRNA fragments in the heatmap. (C,D) tRNA^His and tRNA^Asn fragment coverage from 0Q and 100Q HEK293T cells. The anticodons (GTG for tRNA^His, and GTT for tRNA^Asn) are indicated by a gray bar.