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. 2018 Oct;24(10):1305–1313. doi: 10.1261/rna.067033.118

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© 2018 Wang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Q modification protects cognate tRNA cleavage under arsenite stress and angiogenin treatment in cells. (A) Total small RNA fragment analysis of total cellular RNA isolated from 0Q and 100Q HEK293T cells by SYBR gold staining under conditions of no stress (Mock), plus sodium arsenite (SA), plus recombinant human angiogenin protein (ANG). Total RNA was analyzed on 10% denaturing PAGE containing 7 M urea. Full-length tRNA bands are indicated by a bar on the right. The size region corresponding to tRNA halfmers is also shown in higher contrast on the right. Percentages of total tRNA halfmer sized fragments relative to full-length tRNA are shown below each lane. (B) Same as A for HeLa cells. (C) Northern blot analyses of the same samples in A using tRNA^His and tRNA^Asn probes separated by APB-containing gels. tRNA fragments corresponding to the halfmers are indicated by a bar on the right. (D) Same as C for HeLa cells. (E) Heatmap of tRNA^His and tRNA^Asn halfmer fragment products for 0Q and 100Q cells. The scale bar shows the percent product under these conditions relative to full-length tRNA on the same northern blot.