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. 2018 Oct;24(10):1377–1389. doi: 10.1261/rna.066787.118

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© 2018 Hanson et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Codon optimality contributes to the rate of His3 protein production during ³⁵S-methionine/cysteine labeling. (A) A representative image showing levels of ³⁵S-methionine/cysteine-labeled His3 protein at each time point following addition of ³⁵S-methionine/cysteine to S. cerevisiae cells at mid-log phase. His3 protein expressed from the 0% and 100% optimal HIS3 constructs is presented. (B) Graphical representation of the increase in ³⁵S-methionine/cysteine-labeled His3 protein abundance over time, relative to the protein abundance at the 2 min time point, for each of the 0%–100% optimal HIS3 constructs. (C) The slope of each curve in Figure 5B, normalized to the slope for the 0% optimal construct, is plotted as a measure of the relative rate of ³⁵S incorporation per minute for each HIS3 construct.