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Terpene synthase activity in crude protein extracts of M. histrionica by sex (A) and tissue (B). (A) Tissue of the abdominal cuticle of mature male or female M. histrionica was homogenized in assay buffer and assayed with 100 μM (E,E)-FPP. (B) Different tissues from mature male M. histrionica were homogenized in assay buffer and assayed with 50 μM (E,E)-FPP. Volatile products were extracted with an equal volume of hexane and analyzed with GC-MS. 3, sesquipiperitol; far, (E,E)-farnesol.
