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. 2018 Aug 27;115(37):E8642–E8651. doi: 10.1073/pnas.1717594115

Fig. 6.

Fig. 6.

Electron micrographs of Arp2/3 complex with GST-VCA. Samples of 50 nM Arp2/3 complex, 0.25 µM GST-VCA, and 0.2 mM ATP were negatively stained, imaged by transmission EM, and sorted into classes. (A and B) Analysis by calculating 70 class averages with the ISAC module in EMAN2/Sparx. (A) Top 24 class averages. (B) Angular distribution of GST around Arp2/3 complex with numbers of class averages on the radial axis. (C–E) Two-dimensional classification of the same dataset with Relion yields 11 well-resolved class averages. (C) One example of six class averages including 7,187 (61%) particles that appear like Arp2/3 complex without GST-VCA. The arrow points at ArpC5. (D) Two examples of class averages of particles with foreshortened ArpC5 (arrow) including 4,596 (39%) particles. (E) Orientations of three line scans used to measure dimensions of Arp2/3 complex class averages: D, diagonal; H, horizontal; V, vertical. (F) Mean dimensions (±1 SD).