Fig. 7.

DJ-1 protects chromatin from modification by MGO. (A) DJ-1 hydrolyzes the intermediate aminocarbinol product of MGO and Arg. (B) Western blot analysis demonstrates complete knockout of DJ-1 in both WT and GLO1^−/− cell lines (DKO). (C and D) QuARKMod was performed on chromatin fractions isolated from each cohort, demonstrating significant increases in MG-H1 and CEA in DKO cells compared with GLO1^−/− alone. Treatments were provided for 6 h. Data are presented as mean ± SD; n = 3. Statistical significance was determined via two-way ANOVA. ***P < 0.001.