Skip to main content
. 2018 Aug 27;115(37):E8746–E8754. doi: 10.1073/pnas.1806933115

Fig. 5.

Fig. 5.

Phr1 E3 ligase complex targets cytosolic NMNAT2 for degradation. (A and B) Steady-state levels of NMNAT2(C164S/C165S)-Myc in Cas9-expressing DRG sensory neurons transduced with the indicated sgRNAs, with quantification. A one-way ANOVA was performed with Bonferroni post hoc correction for multiple comparisons, where **P < 0.01, ***P < 0.005, and ****P < 0.0001 for the indicated groups (n = 3). Ctrl, control. (C and D) Turnover of NMNAT2(C164S/C165S)-Myc was assessed in Cas9-expressing DRG sensory neurons treated with cycloheximide (CHX) at the indicated time points, with quantification. Statistical comparisons were performed with t tests, where *P < 0.05 (n = 5). (E and F) Turnover of endogenous SCG10 was assessed in Cas9-expressing DRG sensory neurons, with quantification. No statistically significant differences were observed at any time point (n = 3). (G and H) Steady-state levels of SCG10 (C22S/C24S)-GFP were detected from Cas9-expressing DRG sensory neurons in the presence of the indicated sgRNAs (n = 3). n.s., not significant. Error bars represent SEM.