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. 2018 Aug 23;115(37):E8660–E8667. doi: 10.1073/pnas.1803725115

Fig. 2.

Fig. 2.

NEAT1 is induced in response to SM phenotypic switching in vitro. (A) The qRT-PCR was performed to evaluate NEAT1 expression using the aortic tissues either from the freshly isolated rat thoracic aorta (0 h) or rat thoracic aorta cultured in 20% FBS for the times as indicated; n = 4. *P < 0.05. (B) The qRT-PCR was performed to assess NEAT1 expression in fresh rat aortic tissue or second passage enzymatically dispersed rat aortic VSMCs; n = 4. *P < 0.05. (C) Rat primary aortic VSMCs were treated with PDGF-BB (25 ng/mL) for 8 h or 24 h as indicated, and NEAT1 expression was measured by qRT-PCR; n = 3. *P < 0.05. (D) Rat aortic SMCs were treated without (Left) or with (Middle) PDGF-BB (25 ng/mL) for 24 h, and then RNA-FISH assays were performed to visualize NEAT1 expression. Arrows indicate the representative NEAT1 signal. Cells treated with PDGF-BB and hybridized with NEAT1 sense (S) probe served as the negative control (Right). (E) The numbers of RNA-FISH NEAT1 foci per cell with or without PDGF-BB treatment in HCASMCs (in D) were manually counted and plotted; n = 3. *P < 0.05.