Figure 6.

Effects of soluble NID1 on PDGF-DD-induced cytokine production in Bw-NKp44 cells and human polyclonal NK cells. (a) Bw-NKp44 and Bw-NKp30 cells were left untreated (white bars) or were pre-treated (grey bars) as indicated for 1 h at 37°C and subsequently incubated either on anti-NKp44 or anti-NKp30 mAb-coated plates, respectively, or stimulated with rPDGF-DD at 250 and 25 ng/ml for 20 h. IL-2 release in the SN was evaluated by ELISA. The background (from GAM-stimulated or unstimulated cells) was subtracted for each value. Data are medians of duplicates ± interquartile range and are the pooled results of three independent experiments. *p = 0.0152, **p = 0.0022, ns>0.9999, by two-tailed Mann-Whitney test. (b) Polyclonal NK cell lines were incubated with SN derived from wild type or NID1-transfected K562 cells and cultured for 20 h at 37°C in the presence of rPDGF-DD at 250 and 50 ng/ml. IFN-γ release in the SN was evaluated by ELISA. Data are means of six independent experiments ± SEM performed with NK cells from three donors. *p = 0.0156, *p = 0.0325 (K562-SN vs. K562-SN-NID1 PDGF-DD 50 ng/ml), ns = 0.2188 by one-tailed Wilcoxon test.