Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 11;7(9):e1470730. doi: 10.1080/2162402X.2018.1470730

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

PMC Copyright notice

Figure 9. — Effect of NID1 silencing on NKp44-NID1 interaction in HEK293T cells and in SN derived from NID1-silenced HEK293T cells. (a) NID1 surface expression and NKp44Fc reactivity was analyzed in untransfected HEK293T cells and in HEK293T cells transfected with siRNA-NID1 or siRNA-CTR 48 and 72 h after transfection. Samples were analyzed by flow cytometry. Dotted profiles correspond to isotype control. One representative experiment of three is shown. (b) Western blot analysis of SN derived from HEK293T cells transfected with siRNA-NID1 or siRNA-CTR. Concentrated SN (30 μg for each sample) derived from untransfected HEK293T cells and HEK293T transfected with siRNA-NID1 or siRNA-CTR cultured in protein-free medium were analyzed in SDS-PAGE on a 7.5% polyacrylamide gel; membrane was probed with mouse anti-NID1 mAb or with NKp44Fc molecule followed by the appropriate HRP-conjugated secondary mAb. MW markers (kDa) are indicated on the right. One representative experiment of three is shown.