Table 2.
Association between immune parameters and AJCC (7th edition) T category at diagnosis or presence of metastases.
| Immune parameter | N | Overall | ≤T2b | ≥T3a | P | No metastases | Metastases | P |
|---|---|---|---|---|---|---|---|---|
| Data analyzed as a continuous variablea | ||||||||
| CD3 density | ||||||||
| At periphery | 23 | 2182 (1442) | 1076 (1050) | 2773 (1282) | 0.01 | 1826 (1411) | 2997 (1241) | 0.071 |
| At center | 16 | 456.0 (715.0) | 261.2 (220.9) | 544.5 (848.4) | 0.692 | 275.7 (270.4) | 756.4 (1108.6) | 0.329 |
| Total | 23 | 1577 (1087) | 956 (1059) | 1908 (978) | 0.061 | 1388 (1112) | 2010 (963) | 0.161 |
| CD8 density | ||||||||
| At periphery | 23 | 1162 (900) | 566 (931) | 1481 (726) | 0.006 | 961 (900) | 1623 (768) | 0.071 |
| At center | 16 | 207.6 (337.5) | 57.2 (38.0) | 276.0 (392.2) | 0.396 | 108.3 (174.6) | 373.2 (484.0) | 0.233 |
| Total | 23 | 873 (782) | 531 (945) | 1055 (641) | 0.012 | 743 (760) | 1170 (806) | 0.142 |
| PD-1 density | ||||||||
| At periphery | 23 | 524.5 (531.5) | 243.6 (222.3) | 674.4 (591.6) | 0.024 | 497.2 (575.1) | 587.0 (450.0) | 0.423 |
| At center | 16 | 123.0 (150.1) | 59.3 (93.2) | 151.9 (165.5) | 0.282 | 103.7 (128.0) | 155.1 (190.1) | 0.515 |
| Total | 23 | 383.7 (325.1) | 215.7 (227.1) | 473.3 (340.0) | 0.045 | 373.0 (351.0) | 408.3 (280.2) | 0.593 |
| FOXP3 density | ||||||||
| At periphery | 22 | 438.6 (460.3) | 388.8 (528.4) | 461.8 (443.1) | 0.275 | 464.8 (504.5) | 368.8 (343.7) | 1 |
| At center | 16 | 161.3 (312.2) | 114.7 (143.4) | 182.5 (369.3) | 0.777 | 99.1 (108.8) | 265.0 (500.5) | 0.914 |
| Total | 22 | 346.9 (387.8) | 329.9 (464.7) | 354.9 (364.4) | 0.307 | 358.2 (391.4) | 316.9 (413.0) | 0.825 |
| Data analyzed as a categorical variableb | ||||||||
| PD-L1 status | 23 | 1.000 | 0.371 | |||||
| Negative | 11 (47.8) | 4 (50.0) | 7 (46.7) | 9 (56.2) | 2 (28.6) | |||
| Positive | 12 (52.2) | 4 (50.0) | 8 (53.3) | 7 (43.8) | 5 (71.4) | |||
| CD3 status at periphery | 23 | 0.089 | 0.069 | |||||
| Above median | 12 (52.2) | 2 (25.0) | 10 (66.7) | 6 (37.5) | 6 (85.7) | |||
| Below median | 11 (47.8) | 6 (75.0) | 5 (33.3) | 10 (62.5) | 1 (14.3) | |||
| CD3 status at center | 16 | 1.000 | 0.608 | |||||
| Above median | 8 (50.0) | 2 (40.0) | 6 (54.5) | 4 (40.0) | 4 (66.7) | |||
| Below median | 8 (50.0) | 3 (60.0) | 5 (45.5) | 6 (60.0) | 2 (33.3) | |||
| CD3 status total | 23 | 0.089 | 0.371 | |||||
| Above median | 12 (52.2) | 2 (25.0) | 10 (66.7) | 7 (43.8) | 5 (71.4) | |||
| Below median | 11 (47.8) | 6 (75.0) | 5 (33.3) | 9 (56.2) | 2 (28.6) | |||
| CD8 status at periphery | 23 | 0.009 | 0.069 | |||||
| Above median | 12 (52.2) | 1 (12.5) | 11 (73.3) | 6 (37.5) | 6 (85.7) | |||
| Below median | 11 (47.8) | 7 (87.5) | 4 (26.7) | 10 (62.5) | 1 (14.3) | |||
| CD8 status at center | 16 | 1.000 | 0.608 | |||||
| Above median | 8 (50.0) | 2 (40.0) | 6 (54.5) | 4 (40.0) | 4 (66.7) | |||
| Below median | 8 (50.0) | 3 (60.0) | 5 (45.5) | 6 (60.0) | 2 (33.3) | |||
| CD8 status total | 23 | 0.089 | 0.069 | |||||
| Above median | 12 (52.2) | 2 (25.0) | 10 (66.7) | 6 (37.5) | 6 (85.7) | |||
| Below median | 11 (47.8) | 6 (75.0) | 5 (33.3) | 10 (62.5) | 1 (14.3) | |||
| PD-1 status at periphery | 23 | 0.009 | 0.371 | |||||
| Above median | 12 (52.2) | 1 (12.5) | 11 (73.3) | 7 (43.8) | 5 (71.4) | |||
| Below median | 11 (47.8) | 7 (87.5) | 4 (26.7) | 9 (56.2) | 2 (28.6) | |||
| PD-1 status at center | 16 | 0.308 | 1.000 | |||||
| Above median | 7 (43.8) | 1 (20.0) | 6 (54.5) | 4 (40.0) | 3 (50.0) | |||
| Below median | 9 (56.2) | 4 (80.0) | 5 (45.5) | 6 (60.0) | 3 (50.0) | |||
| PD-1 status total | 23 | 0.089 | 1.000 | |||||
| Above median | 12 (52.2) | 2 (25.0) | 10 (66.7) | 8 (50.0) | 4 (57.1) | |||
| Below median | 11 (47.8) | 6 (75.0) | 5 (33.3) | 8 (50.0) | 3 (42.9) | |||
| FOXP3 status at periphery | 22 | 0.381 | 1.000 | |||||
| Above median | 10 (45.5) | 2 (28.6) | 8 (53.3) | 7 (43.8) | 3 (50.0) | |||
| Below median | 12 (54.5) | 5 (71.4) | 7 (46.7) | 9 (56.2) | 3 (50.0) | |||
| FOXP3 status at center | 16 | 1.000 | 0.633 | |||||
| Above median | 7 (43.8) | 2 (40.0) | 5 (45.5) | 5 (50.0) | 2 (33.3) | |||
| Below median | 9 (56.2) | 3 (60.0) | 6 (54.5) | 5 (50.0) | 4 (66.7) | |||
| FOXP3 status total | 22 | 0.381 | 0.646 | |||||
| Above median | 10 (45.5) | 2 (28.6) | 8 (53.3) | 8 (50.0) | 2 (33.3) | |||
| Below median | 12 (54.5) | 5 (71.4) | 7 (46.7) | 8 (50.0) | 4 (66.7) | |||
aValues are mean (standard deviation) unless otherwise specified. P values are based on Wilcoxon rank-sum test for continuous variables.
bValues are number (percentage) unless otherwise specified. P values are based on Fisher’s exact test for categorical variables.