Table 1. Substrate specificity of pxGSTu1.
| Substrate | Concentration (mM) | Activity (µmol/min/mg) | Wavelength (nm) | Δε (/mM/cm) |
|---|---|---|---|---|
| CDNB | 1.0 | 0.041 | 340 | 9.6 |
| EPNP | 1.0 | NA | 260 | 0.5 |
| 4NBC | 1.0 | NA | 310 | 1.9 |
| 4NPB | 1.0 | NA | 310 | 1.2 |
| 4HNE | 0.1 | NA | 224 | 13.8 |
| ECA | 0.2 | 0.13 | 270 | 5.0 |
| 4NPA | 1.0 | NA | 400 | 8.3 |
| H2O2 | 0.2 | NA | 340 | −6.2 |
Activity was measured at pH 8 in the presence of 5 mM GSH. Data are expressed as means of three independent experiments. NA represents no activity. Wavelength and Δε represent maximum wavelength of the absorption and molecular coefficient, respectively.