Skip to main content
. 2018 Aug 20;43(3):173–179. doi: 10.1584/jpestics.D18-003

Fig. 5. Treatment with GR24 triggered cell death, which is alleviated by co-treatment with Glc. (A) Trypan blue staining was performed to detect the cell death of leaf segments with treatment of GR24 (2 µM) or co-treatment of GR24 (2 µM) and Glc (0.8%) under a light or dark condition on day 14. (B) Electrophoresis was performed to check the degradation of genomic DNA. (C) Homology analysis of cDNAs from B. oldhamii and a tomato (Solanum lycopersicum). Bo_eIF-5A-like displays over 98% similarity to tomato_eIF-5A. (D) Real-time RT-PCR analysis was performed to monitor transcript levels of the Bo_eIF-5A-like of leaf segments with a treatment of GR24 (2 µM) or co-treatment of GR24 (2 µM) and Glc (0.8%) under a dark condition. Error bars indicate±SD, n=3.

Fig. 5. Treatment with GR24 triggered cell death, which is alleviated by co-treatment with Glc. (A) Trypan blue staining was performed to detect the cell death of leaf segments with treatment of GR24 (2 µM) or co-treatment of GR24 (2 µM) and Glc (0.8%) under a light or dark condition on day 14. (B) Electrophoresis was performed to check the degradation of genomic DNA. (C) Homology analysis of cDNAs from B. oldhamii and a tomato (Solanum lycopersicum). Bo_eIF-5A-like displays over 98% similarity to tomato_eIF-5A. (D) Real-time RT-PCR analysis was performed to monitor transcript levels of the Bo_eIF-5A-like of leaf segments with a treatment of GR24 (2 µM) or co-treatment of GR24 (2 µM) and Glc (0.8%) under a dark condition. Error bars indicate±SD, n=3.